Targeted complete NGS and quality control of transgenes and integration sites in upstream cell & gene therapy manufacturing

In the upstream cell line development and manufacturing process, the virus producing cell lines are generated. To analyze and QC transgenic cell lines, Southern Blot and FISH analyses are often used. In this appnote, we discuss TLA-based transgene and integration site sequencing as a cost-effective, high quality alternative to conventional approaches for the quality control of transgenic cells.


  • Cergentis’ TLA technology is a powerful tool to completely sequence transgenes and their integration sites.
  • TLA analyses are used in the quality control of cell lines, for clone selection, and for the assessment of the clonality and genetic stability of cell lines used for the manufacturing of gene therapy products.
Application note - cell and gene therapy upstream