Application Note


Targeted complete next generation sequencing and quality control of transgenes and integration sites in CHO cell line development

Chinese hamster ovary (CHO) cells are widely used for the production of recombinant proteins and therapeutic antibodies. The efficient generation of high-quality CHO cell lines is a critical step in the development and production of (candidate) drugs. To analyze the genetics of the generated cell lines, Southern Blot and FISH analyses are often used. However, generated data is incomplete and can be hard to interpret. Download this appnote and discover the power of TLA in sequencing transgenes and their integration sites.


  • TLA analyses are used in the development and quality control of cell lines, for clone selection, and for the assessment of clonality and genetic stability.
  • Cergentis' TLA technology is a powerful tool to completely sequence transgenes and their integration sites.


Application note - CHO cell line development